événement
Défense de thèse de doctorat en sciences biologiques
Delayed effect of early-life exposure to 17-α-ethinylestradiol on the DNA methylation landscape, proteome and physiology of the self-fertilizing mangrove rivulus Kryptolebias marmoratus
Catégorie :
défense de thèse
Date : 02/05/2018 15:00 - 02/05/2018 17:00
Lieu : Amphithéâtre L12, rue Grafé, 5000 Namur
Orateur(s) : Anne-Sophie VOISIN
Organisateur(s) : Frédéric SILVESTRE
Date : 02/05/2018 15:00 - 02/05/2018 17:00
Lieu : Amphithéâtre L12, rue Grafé, 5000 Namur
Orateur(s) : Anne-Sophie VOISIN
Organisateur(s) : Frédéric SILVESTRE
Jury
Caroline NIEBERDING (UCL), Ryan EARLEY (University of Alabama), Marie-Agnès COUTELLEC (INRA, Rennes), Karine VAN DONINCK (UNamur), présidente, Victoria SUAREZ-ULLOA (UNamur), Frédéric SILVESTRE, promoteur (UNamur)
Résumé
Endocrine disrupting chemicals (EDCs) can
mimic or interfere with endogenous hormone processes that are crucial
for the normal development of organisms. Nevertheless, they are omnipresent in everyday products and pollute our aquatic environments.
I investigated the delayed effects of ethinylestradiol (EE2), a
synthetic estrogen used in oral contraceptives, on the development of mangrove rivulus, Kryptolebias marmoratus. This fish is the world’s only known self-fertilizing hermaphrodite, which makes it possible to produce genetically identical individuals and focus on environmental effects.
Months after exposure to EE2, hatchlings showed differences in compensatory growth, fecundity and steroid hormone levels. To further understand the underlying molecular mechanisms, I measured the proteome and epigenome (methylome) in brain, liver and gonads of adult fish. Usually, estrogen-responsive pathways, such as lipid metabolism, inflammation, and the innate immune system are affected after acute exposure to EE2. I could show for the first time, that these were also affected months after exposure.
My results provide further evidence for the role of DNA methylation in mediating long-term changes in cellular processes resulting from early-life exposures.
Months after exposure to EE2, hatchlings showed differences in compensatory growth, fecundity and steroid hormone levels. To further understand the underlying molecular mechanisms, I measured the proteome and epigenome (methylome) in brain, liver and gonads of adult fish. Usually, estrogen-responsive pathways, such as lipid metabolism, inflammation, and the innate immune system are affected after acute exposure to EE2. I could show for the first time, that these were also affected months after exposure.
My results provide further evidence for the role of DNA methylation in mediating long-term changes in cellular processes resulting from early-life exposures.
La défense est publique
Télecharger :
vCal